Decide exactly what you expect to win and why, such as a benefit-led line under forty-five characters outperforming a playful pun for new subscribers. Write the predicted direction, the decision rule, and the stop condition, so you resist moving goalposts once the first positive signals appear.
Use a sample-size calculator or your ESP’s guidance to avoid underpowered tests that crown random noise as a winner. Target consistent send windows across time zones, cap the decision period to one or two days, and segment out dormant addresses that skew engagement baselines.
Because many devices auto-load pixels, treat open rate as a directional signal, not an exact truth. Compare variants within the same domain mix, check click-to-open, and confirm the winner on the next campaign, preventing one lucky send time from steering your subject direction for months.
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